If you want to start migrating over to the mice, we’re actually gonna start class with hands-on demonstration by the mouse cages I am Tom Gustad. I’m an instructor in the department of veterinary and microbiological
sciences at NDSU. So, for this exercise each group of four, or group of two, is going to get a cage with two mice. When you do your injection today you’re going to get one 1 cc syringe with a 22 gauge needle. You’re going to first fill it with 0.5 mL of
your PBS that you made last thursday and do an IP injection of the control mouse.
And then, using the same syringe, fill it with 0.5 cc/0.5 mL of the washed
sheep red blood cells. Make sure you mix it up good Make sure you mix it up good, those sheep red blood cells settle out. And do an IP injected of that into the
experimental mouse, and that’ll be the routine throughout the exercise. and that’ll be the routine throughout the exercise. Teaching is very exciting, you might say, “doesn’t it get monotonous
with teaching the same material each year?” Well, you know, I like to bring new material in
on a regular basis, but the thing is the students. The students. You always have a
new crop of students that are exciting and inquisitive and they really keep you
motivated to keep teaching and keep trying to improve your teaching. So, watch closely. You grab the most by the tail.
Short “L”. That’s the key. Pretend you’re a raptor (bird) and you’re gonna eat that mouse for supper. So you’re gonna come down – “boom”. Now, we’re doing an IP injection which means I have to go through both the hide, the outer
epidermis, which would be like my lab coat and the peritoneum underneath. One of the two
laboratory mouse exercises that the students do that last most of the
semester; they revolve around vaccinology really. They’re immunizing these mice with an antigen in hopes of eliciting an immune reaction. And then at the end of the project they will evaluate that immune reaction with with a challenge study using live bacteria. Instructor: So do the, do the short “L”. Student: That’s what I tried! Instructor: And just, when you go… Student: I’m just too… Instructor: See I haven’t even squeeze my hands together yet, I’m just holding him agains the cage lid. Now watch my fingers, my hands, then I close them up. Student: Okay.
Instructor: Okay. And not only do they learning about
vaccinology and eliciting an immune response, but they also learn some
valuable skills that every immunologist needs. That is, how to handle mice; how to
restrain them, how to inject them, all the while without injuring the mouse. And, to give them some information on how vaccines work. So, watch my fingers. First I just, I’m not
even, I haven’t even squeezed my fingers together yet. I’m just holding him down
so he can’t turn around and bite me. Now, watch my fingers and then I squeeze. Student: I don’t think it’ll be that bad once I just do it. I would tell them, and any student, as far as to achieved academic success, to take it seriously. I like to tell my advisees,
to think of your college experience like you would a job. I mean, you don’t decide at the last minute not to show up for work so don’t decide not to show up